Part:BBa_K1919003:Design

T7 promotor (under the control of lacI) and downstream CecropinXJ

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2008
  Illegal BamHI site found at 2051
  Illegal XhoI site found at 2252
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1418
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The coding sequence was optimized for E.coli expression using JCat http://www.jcat.de/. The single stop codon was replaced by double TAA stop codon.

Source

The original sequence of CecropinXJ comes from GenBank NM_001043995.1. The sample used for experiments was artificially synthesized by Tsingke.